The Slade Lab
Papers
Investigating how bacteria influence the tumor microenvironment.
Publications
All publications on
Blue names indicate members of The Slade Lab
Michael A. Casasanta*, Christopher C. Yoo*, Barath Udayasuryan*, Blake E. Sanders, Ariana Umaña, Yao Zhang, Huaiyao Peng, Alison J. Duncan, Yueying Wang, Liwu Li, Scott S. Verbridge, Daniel J. Slade#. 2020. Science Signaling 21 Jul 2020: Vol. 13, Issue 641, eaba9157. Link
The Cancer Microbiome: Distinguishing Direct and Indirect Effects Requires a Systemic View.
Joao B Xavier, Vincent B Young, Joseph Skufca, Fiona Ginty, Traci Testerman, Alexander T Pearson, Paul Macklin, Amir Mitchell, Ilya Shmulevich, Lei Xie , J Gregory Caporaso, Keith A Crandall, Nicole L Simone, Filipa Godoy-Vitorino, Timothy J Griffin, Katrine L Whiteson, Heather H Gustafson, Daniel J Slade, Thomas M Schmidt, Marina R S Walther-Antonio, Tal Korem, Bobbie-Jo M Webb-Robertson, Mark P Styczynski, W Evan Johnson, Christian Jobin, Jason M Ridlon, Andrew Y Koh, Michael Yu, Libusha Kelly, Jennifer A Wargo. Trends in Cancer. 2020 Mar;6(3):192-204. Link
Zheng L, Nagar M, Maurais AJ, Slade DJ, Parelkar SS, Coonrod SA, Weerapana E, Thompson PR. Biochemistry. 2019 Jul 9;58(27):3042-3056. Link.
Utilizing Whole Fusobacterium Genomes To Identify, Correct, and Characterize Potential Virulence Protein Families
Umana, A., Sanders, B.E., Yoo, C.C., Casasanta, M.A., Udayasuryan, B., Verbridge, S.S., Slade, D.J.# 2019. J Bacteriol. Link.
Complete genome sequence of Fusobacterium necrophorum subsp. necrophorum ATCC 25286.
Umana, A., Lemkul, J.A., Slade, D.J.# Microbiol Resour Announc. 2019. Volume 8 Issue 8 e00025-19. Link
Microfluidics in Microbiome & Cancer Research.
Udayasuryan, B., Slade, D.J., Verbridge, S.S. Nanotechnology and Microfluidics. Wiley. 2019. Accepted.
A Vector Suite for the Overexpression and Purification of Tagged outer Membrane, Periplasmic, and Secreted Proteins in E. coli.
Casasanta, M.A., Slade, D.J.# Mouse Models of Innate Immunity: Methods and Protocols, Methods in Molecular Biology. Vol. 1960. Link.
Sanders, B.E., Umana, A., Lemkul, J.A., Slade, D.J.# mSphere. 2018 Jul 5;3(4). Link.
Fusobacterium Genomics Using MinION and Illumina Sequencing Enables Genome Completion and Correction.
Michelle Todd, S., Settlage, R.E., Lahmers, K.K., Slade, D.J.# mSphere. 2018 Jul 5;3(4). Link.
Biological Studies and Target Engagement of the 2- C-Methyl-d-Erythritol 4-Phosphate Cytidylyltransferase (IspD)-Targeting Antimalarial Agent (1 R,3 S)-MMV008138 and Analogs.
Ghavami M, Merino EF, Yao ZK, Elahi R, Simpson ME, Fernández-Murga ML, Butler JH, Casasanta MA, Krai PM, Totrov MM, Slade DJ, Carlier PR, Cassera MB. ACS Infect Dis. 2018 Apr 13;4(4):549-559. Link.
Casasanta MA, Yoo CC, Smith HB, Duncan AJ, Cochrane K, Varano AC, Allen-Vercoe E, Slade DJ.# J Biol Chem. 2017 Dec 8;292(49):20240-20254.
Link.
N-(3-oxododecanoyl)-L-homoserine lactone interactions in the breast tumor microenvironment: Implications for breast cancer viability and proliferation in vitro.
Balhouse BN, Patterson L, Schmelz EM, Slade DJ, Verbridge SS. PLoS One. 2017 Jul 10;12(7) Link.
Enhanced Mucosal Defense and Reduced Tumor Burden in Mice with the Compromised Negative Regulator IRAK-M.
Rothschild DE, Zhang Y, Diao N, Lee CK, Chen K, Caswell CC, Slade DJ, Helm RF, LeRoith T, Li L, Allen IC. EBioMedicine. 2017 Feb;15:36-47. Link.
Lewallen DM, Bicker KL, Subramanian V, Clancy KW, Slade DJ, Martell J, Dreyton CJ, Sokolove J, Weerapana E, Thompson PR. ACS Chem Biol. 2015 Nov 20;10(11):2520-8. Link.
Inhibition of PAD4 activity is sufficient to disrupt mouse and human NET formation.
Lewis HD, Liddle J, Coote JE, Atkinson SJ, Barker MD, Bax BD, Bicker KL, Bingham RP, Campbell M, Chen YH, Chung CW, Craggs PD, Davis RP, Eberhard D, Joberty G, Lind KE, Locke K, Maller C, Martinod K, Patten C, Polyakova O, Rise CE, Rüdiger M, Sheppard RJ, Slade DJ, Thomas P, Thorpe J, Yao G, Drewes G, Wagner DD, Thompson PR, Prinjha RK, Wilson DM. Nat Chem Biol. 2015 Mar;11(3):189-91. Link.
Protein arginine deiminase 2 binds calcium in an ordered fashion: implications for inhibitor design.
Slade DJ, Fang P, Dreyton CJ, Zhang Y, Fuhrmann J, Rempel D, Bax BD, Coonrod SA, Lewis HD, Guo M, Gross ML, Thompson PR. ACS Chem Biol. 2015 Apr 17;10(4):1043-53. Link.
Slade DJ, Horibata S, Coonrod SA, Thompson PR. Bioessays. 2014 Aug;36(8):736-40. Link.
Pluripotency: citrullination unravels stem cells.
Slade DJ, Subramanian V, Thompson PR. Nat Chem Biol. 2014 May;10(5):327-8. Link.
Chemical and biological methods to detect post-translational modifications of arginine.
Slade DJ, Subramanian V, Fuhrmann J, Thompson PR. Biopolymers. 2014 Feb;101(2):133-43. Link.
Rohrbach AS, Slade DJ, Thompson PR, Mowen KA. Front Immunol. 2012 Nov 29;3:360. Link.
Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor α target gene activation.
Zhang X, Bolt M, Guertin MJ, Chen W, Zhang S, Cherrington BD, Slade DJ, Dreyton CJ, Subramanian V, Bicker KL, Thompson PR, Mancini MA, Lis JT, Coonrod SA. Proc Natl Acad Sci U S A. 2012 Aug 14;109(33):13331-6. doi: 10.1073/pnas.1203280109. Link.
Genetic reporter system for positioning of proteins at the bacterial pole.
Fixen KR, Janakiraman A, Garrity S, Slade DJ, Gray AN, Karahan N, Hochschild A, Goldberg MB. mBio. 2012 Feb 28;3(2). Link.
Slade DJ, Lovelace LL, Chruszcz M, Minor W, Lebioda L, Sodetz JM. J Mol Biol. 2008 May 30;379(2):331-42. doi: 10.1016/j.jmb.2008.03.061. Link.
Crystal structure of complement protein C8gamma in complex with a peptide containing the C8gamma binding site on C8alpha: implications for C8gamma ligand binding.
Lovelace LL, Chiswell B, Slade DJ, Sodetz JM, Lebioda L. Mol Immunol. 2008 Feb;45(3):750-6. Link.
Chiswell B, Slade DJ, Sodetz JM. Biochim Biophys Acta. 2006 Sep;1764(9):1518-24. Link.
Functional studies of the MACPF domain of human complement protein C8alpha reveal sites for simultaneous binding of C8beta, C8gamma, and C9.
Slade DJ, Chiswell B, Sodetz JM. Biochemistry. 2006 Apr 25;45(16):5290-6. Link.
Michael A. Casasanta, Christopher C. Yoo, Barath Udayasuryan, Blake E. Sanders, Ariana Umaña, Yao Zhang, Huaiyao Peng, Alison J. Duncan, Yueying Wang, Liwu Li, Scott S. Verbridge, Daniel J. Slade#. 2020. bioRxiv. Now published in Science Signaling. Link.
Reevaluating the Fusobacterium Virulence Factor Landscape.
Umana, A., Sanders, B.E., Yoo, C.C., Casasanta, M.A., Udayasuryan, B., Verbridge, S.S., Slade, D.J.# 2019. bioRxiv. Now published in J Bacteriol. Link.
FusoPortal: An interactive repository of hybrid MinION sequenced Fusobacterium genomes improves gene identification and characterization.
Sanders, B.E., Umana, A., Lemkul, J.A., Slade, D.J.#, 2018. bioRxiv. Now published in mSphere. Link.
Fusobacterium genomics using MinION and Illumina sequencing enables genome completion and correction.
Todd, S.M., Settlage, R.E., Lahmers, K.K., Slade, D.J.#, 2018. bioRxiv. Now published in mSphere. Link.
FplA From Fusobacterium nucleatum Is A Type Vd Autotransporter Phospholipase With A Proposed Role In Altered Host Signaling And Evasion Of Autophagy.
Casasanta, M.A., Yoo, C.C., Smith, H.B., Duncan, A.J., Cochrane, K., Varano, A.C., Allen-Vercoe, E., Slade, D.J.#, 2017. bioRxiv. Now published in Journal of Biological Chemistry. Link.
N-(3-oxododecanoyl)-L-homoserine lactone interactions in the breast tumor microenvironment: Implications for breast cancer viability and proliferation in vitro.
Balhouse B.N., Patterson L., Schmelz E.M., Slade D.J., Verbridge S.S. 2017. bioRxiv. Now published in PLoS ONE. Link.
2020
Fusobacterium nucleatum host cell binding and invasion induces IL-8 and CXCL1 secretion that drives colorectal cancer cell migration.Michael A. Casasanta*, Christopher C. Yoo*, Barath Udayasuryan*, Blake E. Sanders, Ariana Umaña, Yao Zhang, Huaiyao Peng, Alison J. Duncan, Yueying Wang, Liwu Li, Scott S. Verbridge, Daniel J. Slade#. 2020. Science Signaling 21 Jul 2020: Vol. 13, Issue 641, eaba9157. Link
The Cancer Microbiome: Distinguishing Direct and Indirect Effects Requires a Systemic View.
Joao B Xavier, Vincent B Young, Joseph Skufca, Fiona Ginty, Traci Testerman, Alexander T Pearson, Paul Macklin, Amir Mitchell, Ilya Shmulevich, Lei Xie , J Gregory Caporaso, Keith A Crandall, Nicole L Simone, Filipa Godoy-Vitorino, Timothy J Griffin, Katrine L Whiteson, Heather H Gustafson, Daniel J Slade, Thomas M Schmidt, Marina R S Walther-Antonio, Tal Korem, Bobbie-Jo M Webb-Robertson, Mark P Styczynski, W Evan Johnson, Christian Jobin, Jason M Ridlon, Andrew Y Koh, Michael Yu, Libusha Kelly, Jennifer A Wargo. Trends in Cancer. 2020 Mar;6(3):192-204. Link
2019
Calcium Regulates the Nuclear Localization of Protein Arginine Deiminase 2.Zheng L, Nagar M, Maurais AJ, Slade DJ, Parelkar SS, Coonrod SA, Weerapana E, Thompson PR. Biochemistry. 2019 Jul 9;58(27):3042-3056. Link.
Utilizing Whole Fusobacterium Genomes To Identify, Correct, and Characterize Potential Virulence Protein Families
Umana, A., Sanders, B.E., Yoo, C.C., Casasanta, M.A., Udayasuryan, B., Verbridge, S.S., Slade, D.J.# 2019. J Bacteriol. Link.
Complete genome sequence of Fusobacterium necrophorum subsp. necrophorum ATCC 25286.
Umana, A., Lemkul, J.A., Slade, D.J.# Microbiol Resour Announc. 2019. Volume 8 Issue 8 e00025-19. Link
Microfluidics in Microbiome & Cancer Research.
Udayasuryan, B., Slade, D.J., Verbridge, S.S. Nanotechnology and Microfluidics. Wiley. 2019. Accepted.
A Vector Suite for the Overexpression and Purification of Tagged outer Membrane, Periplasmic, and Secreted Proteins in E. coli.
Casasanta, M.A., Slade, D.J.# Mouse Models of Innate Immunity: Methods and Protocols, Methods in Molecular Biology. Vol. 1960. Link.
2018
FusoPortal: an Interactive Repository of Hybrid MinION-Sequenced Fusobacterium Genomes Improves Gene Identification and Characterization.Sanders, B.E., Umana, A., Lemkul, J.A., Slade, D.J.# mSphere. 2018 Jul 5;3(4). Link.
Fusobacterium Genomics Using MinION and Illumina Sequencing Enables Genome Completion and Correction.
Michelle Todd, S., Settlage, R.E., Lahmers, K.K., Slade, D.J.# mSphere. 2018 Jul 5;3(4). Link.
Biological Studies and Target Engagement of the 2- C-Methyl-d-Erythritol 4-Phosphate Cytidylyltransferase (IspD)-Targeting Antimalarial Agent (1 R,3 S)-MMV008138 and Analogs.
Ghavami M, Merino EF, Yao ZK, Elahi R, Simpson ME, Fernández-Murga ML, Butler JH, Casasanta MA, Krai PM, Totrov MM, Slade DJ, Carlier PR, Cassera MB. ACS Infect Dis. 2018 Apr 13;4(4):549-559. Link.
2017
A chemical and biological toolbox for Type Vd secretion: Characterization of the phospholipase A1 autotransporter FplA from Fusobacterium nucleatum.Casasanta MA, Yoo CC, Smith HB, Duncan AJ, Cochrane K, Varano AC, Allen-Vercoe E, Slade DJ.# J Biol Chem. 2017 Dec 8;292(49):20240-20254.
Link.
N-(3-oxododecanoyl)-L-homoserine lactone interactions in the breast tumor microenvironment: Implications for breast cancer viability and proliferation in vitro.
Balhouse BN, Patterson L, Schmelz EM, Slade DJ, Verbridge SS. PLoS One. 2017 Jul 10;12(7) Link.
Enhanced Mucosal Defense and Reduced Tumor Burden in Mice with the Compromised Negative Regulator IRAK-M.
Rothschild DE, Zhang Y, Diao N, Lee CK, Chen K, Caswell CC, Slade DJ, Helm RF, LeRoith T, Li L, Allen IC. EBioMedicine. 2017 Feb;15:36-47. Link.
2015
Chemical Proteomic Platform To Identify Citrullinated Proteins.Lewallen DM, Bicker KL, Subramanian V, Clancy KW, Slade DJ, Martell J, Dreyton CJ, Sokolove J, Weerapana E, Thompson PR. ACS Chem Biol. 2015 Nov 20;10(11):2520-8. Link.
Inhibition of PAD4 activity is sufficient to disrupt mouse and human NET formation.
Lewis HD, Liddle J, Coote JE, Atkinson SJ, Barker MD, Bax BD, Bicker KL, Bingham RP, Campbell M, Chen YH, Chung CW, Craggs PD, Davis RP, Eberhard D, Joberty G, Lind KE, Locke K, Maller C, Martinod K, Patten C, Polyakova O, Rise CE, Rüdiger M, Sheppard RJ, Slade DJ, Thomas P, Thorpe J, Yao G, Drewes G, Wagner DD, Thompson PR, Prinjha RK, Wilson DM. Nat Chem Biol. 2015 Mar;11(3):189-91. Link.
Protein arginine deiminase 2 binds calcium in an ordered fashion: implications for inhibitor design.
Slade DJ, Fang P, Dreyton CJ, Zhang Y, Fuhrmann J, Rempel D, Bax BD, Coonrod SA, Lewis HD, Guo M, Gross ML, Thompson PR. ACS Chem Biol. 2015 Apr 17;10(4):1043-53. Link.
2014
A novel role for protein arginine deiminase 4 in pluripotency: the emerging role of citrullinated histone H1 in cellular programming.Slade DJ, Horibata S, Coonrod SA, Thompson PR. Bioessays. 2014 Aug;36(8):736-40. Link.
Pluripotency: citrullination unravels stem cells.
Slade DJ, Subramanian V, Thompson PR. Nat Chem Biol. 2014 May;10(5):327-8. Link.
Chemical and biological methods to detect post-translational modifications of arginine.
Slade DJ, Subramanian V, Fuhrmann J, Thompson PR. Biopolymers. 2014 Feb;101(2):133-43. Link.
2012
Activation of PAD4 in NET formation.Rohrbach AS, Slade DJ, Thompson PR, Mowen KA. Front Immunol. 2012 Nov 29;3:360. Link.
Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor α target gene activation.
Zhang X, Bolt M, Guertin MJ, Chen W, Zhang S, Cherrington BD, Slade DJ, Dreyton CJ, Subramanian V, Bicker KL, Thompson PR, Mancini MA, Lis JT, Coonrod SA. Proc Natl Acad Sci U S A. 2012 Aug 14;109(33):13331-6. doi: 10.1073/pnas.1203280109. Link.
Genetic reporter system for positioning of proteins at the bacterial pole.
Fixen KR, Janakiraman A, Garrity S, Slade DJ, Gray AN, Karahan N, Hochschild A, Goldberg MB. mBio. 2012 Feb 28;3(2). Link.
2008
Crystal structure of the MACPF domain of human complement protein C8 alpha in complex with the C8 gamma subunit.Slade DJ, Lovelace LL, Chruszcz M, Minor W, Lebioda L, Sodetz JM. J Mol Biol. 2008 May 30;379(2):331-42. doi: 10.1016/j.jmb.2008.03.061. Link.
Crystal structure of complement protein C8gamma in complex with a peptide containing the C8gamma binding site on C8alpha: implications for C8gamma ligand binding.
Lovelace LL, Chiswell B, Slade DJ, Sodetz JM, Lebioda L. Mol Immunol. 2008 Feb;45(3):750-6. Link.
2006
Binding of the lipocalin C8gamma to human complement protein C8alpha is mediated by loops located at the entrance to the C8gamma ligand binding site.Chiswell B, Slade DJ, Sodetz JM. Biochim Biophys Acta. 2006 Sep;1764(9):1518-24. Link.
Functional studies of the MACPF domain of human complement protein C8alpha reveal sites for simultaneous binding of C8beta, C8gamma, and C9.
Slade DJ, Chiswell B, Sodetz JM. Biochemistry. 2006 Apr 25;45(16):5290-6. Link.
Preprint History on bioRxiv
Fusobacterium nucleatum host cell binding and invasion induces IL-8 and CXCL1 secretion that drives colorectal cancer cell migration.Michael A. Casasanta, Christopher C. Yoo, Barath Udayasuryan, Blake E. Sanders, Ariana Umaña, Yao Zhang, Huaiyao Peng, Alison J. Duncan, Yueying Wang, Liwu Li, Scott S. Verbridge, Daniel J. Slade#. 2020. bioRxiv. Now published in Science Signaling. Link.
Reevaluating the Fusobacterium Virulence Factor Landscape.
Umana, A., Sanders, B.E., Yoo, C.C., Casasanta, M.A., Udayasuryan, B., Verbridge, S.S., Slade, D.J.# 2019. bioRxiv. Now published in J Bacteriol. Link.
FusoPortal: An interactive repository of hybrid MinION sequenced Fusobacterium genomes improves gene identification and characterization.
Sanders, B.E., Umana, A., Lemkul, J.A., Slade, D.J.#, 2018. bioRxiv. Now published in mSphere. Link.
Fusobacterium genomics using MinION and Illumina sequencing enables genome completion and correction.
Todd, S.M., Settlage, R.E., Lahmers, K.K., Slade, D.J.#, 2018. bioRxiv. Now published in mSphere. Link.
FplA From Fusobacterium nucleatum Is A Type Vd Autotransporter Phospholipase With A Proposed Role In Altered Host Signaling And Evasion Of Autophagy.
Casasanta, M.A., Yoo, C.C., Smith, H.B., Duncan, A.J., Cochrane, K., Varano, A.C., Allen-Vercoe, E., Slade, D.J.#, 2017. bioRxiv. Now published in Journal of Biological Chemistry. Link.
N-(3-oxododecanoyl)-L-homoserine lactone interactions in the breast tumor microenvironment: Implications for breast cancer viability and proliferation in vitro.
Balhouse B.N., Patterson L., Schmelz E.M., Slade D.J., Verbridge S.S. 2017. bioRxiv. Now published in PLoS ONE. Link.
Get in touch
© All rights reserved. Daniel Slade 2020. Design: HTML5 UP modified by Daniel Slade.